Multiplex ELISA probing unveiled higher levels of pro-inflammatory cytokines IL-5, IL-12, GM-CSF and MCP-1 in the cortex of 12-month-old 3xTg-AD mice, compared to NonTg, when expressed as ratios relative to the anti-inflammatory cytokine IL-10 (Figure 6B). This evidence concerns the gene IL10 and Alzheimer disease.