To determine whether the impact of oridonin on tumor growth inhibition was related to caspase-3, caspase-9, NF-κB, Bax, Bcl-2, PARP-1 and cytochrome c, we evaluated the levels of these apoptosis-related proteins in the gallbladder tumor tissues by western blot analysis. This evidence concerns the gene CASP3 and gallbladder neoplasm.