However, more recently, 2% dietary cholesterol in the context of high fat, high fructose diet (where the source of fat was trans-fat) elicited fibrosis in leptin-deficient mice, but not in normal C57BL6 mice [21], suggesting that multiple insults are required in other rodent models for the full spectrum of NASH, not just increased dietary cholesterol [22]. Here, LEP is linked to metabolic dysfunction-associated steatohepatitis.