The disease-mediated increase observed by RNA-Seq analysis in U1 and RP11-462G22.1 was faithfully validated by real-time RT-PCR in the PD leukocytes (Figure 2D, one-tailed t-test p = 0.049 for RP11-462G22.1 and RPL19 as reference gene, non-significant for U1; details under Methods), which further raised the question of the relevance of the observed leukocyte alterations to the PD brain degenerative process. The gene discussed is RPL19; the disease is Parkinson disease.