DNA isolated from 49 tissue samples with no histological evidence for CIN (normal), from 43 samples diagnosed as CIN3 and from 54 cervical cancer tissues was bisulfite-treated and used in qMSP experiments for the five marker regions DLX1, ITGA4, RXFP3, SOX17, and ZNF671. The gene discussed is ITGA4; the disease is cervical squamous intraepithelial neoplasia.