11. The Bentires-Alj group produced the similar results with breast cancer cell lines and primary tumors that amplified and highly expressed ANO1 7. Likewise, knockdown of ANO1 in the ESCC cell lines, KYSE30 and KYSE510 cells significantly inhibited the proliferation of the cells 14. This evidence concerns the gene ANO1 and esophageal squamous cell carcinoma.