To test this hypothesis in the context of breast cancer, here we investigated the effects of restoring Dab2 expression in SK-BR-3 cell (a human breast cancer cell line lacking Dab2 expression) on the TGF-β depletion, and the influence of abnormal TGF-β depletion on the differentiation of Tregs under in vitro conditions mimicking the tumor environment. This evidence concerns the gene TGFB1 and neoplasm.