AKT1 and neoplasm: Total proteins of NIH3T3-flagOVA66 tumor tissues, and corresponding tissues where NIH3T3-mock cells were injected were extracted and then analyzed by western blotting using 4G9, phospho-AKT and phosphor-ERK1/2 antibodies to detect OVA66 expression and phosphorylation of Akt and Erk1/2 (Figure 5C), indicating OVA66 overexpression (confirmed by IHC in Figure S3) in NIH3T3-flagOVA66 formed tumors and aberrantly activated AKT and ERK1/2.