To determine whether TLR4 and chemokine CXCL2 play a role in infection process, Chinese hamsters (n  =  6) were infected with either pathogenic (A. castellanii or A. culbertsoni) or non-pathogenic (A. astronyxis or A. castellanii Neff) strains of Acanthamoeba as described previously [7]. This evidence concerns the gene TLR4 and infection.