IL4 and Increased total eosinophil count: In the Gregory et al (2009) study, adult female BALB/c mice intranasally exposed to 25 μg of HDM protein in saline 5 days per week for 2 weeks had significantly increased airway resistance, lung and BAL eosinophilia and neutrophilia, IL-4, IL-5 and IL-13 compared to controls 24 hours after the final exposure [60].