The ESX1 region of Mtb is also associated with increased cellular necrosis and cellular egress at 72 h post-infection [5] so we assessed whether fractalkine plays a role in cellular necrosis at this time point using an anti-fractalkine monoclonal antibody, recombinant fractalkine and Triton-X 100 induction of necrosis as a positive control. Here, CX3CL1 is linked to infection.