MMP2 and Salla disease: Expression of MMP-2 and MT1-MMP proteins from sections and supernates of 3-D culture samples of GBC-SD cells in vitro were examined by SABC and ELISA, and expression of PI3-K, MMP-2, MT1-MMP and Ln-5γ2 at protein and mRNA levels from sections of GBC-SD xenografts in vivo were determined by SABC, indirect immunofluorescence, Western blotting and RT-PCR.