CASP1 and infection: To further analyse the underlying mechanisms of the different courses of cell death in caspase-1/11 KO and wild-type macrophages, we determined the activation of inflammatory caspase-1, classical apoptotic caspases as well as the phosphorylation of apoptosis-related kinases at different time points after infection with B. pseudomallei E8 or B. thailandensis E264 by immunoblotting.