Rapid NLRC4- and caspase-1-dependent cell death in wild-type macrophages at high MOI led to high LDH release within the first hours after infection compared to NLRC4 and caspase-1/11 knockout macrophages, which exhibited signs of cell death as recently as 24 hours (Figures S7A, 2C). This evidence concerns the gene NLRC4 and infection.