In contrast, our study showed that infection of wild-type (Figure 1) and NLRP3 knockout macrophages (Figure 5B) but not NLRC4-deficient macrophages (Figure 5A) with B. pseudomallei E8 leads to activation of caspase-1 and downstream signalling pathways at 1.5 hours after infection. The gene discussed is NLRP3; the disease is infection.