Therefore, the purpose of our study was to evaluate the effects of EA-like stimulation at the Baihui and Dazhui acupoints (EA at acupoints) after 15 min of cerebral ischemia followed by 7 d of reperfusion, and to elucidate the regulatory mechanisms involved in the S100B-mediated signaling pathway in the periinfarct area following ischemia-reperfusion (I/R) injury. The gene discussed is S100B; the disease is Cerebral ischemia.