On the basis of these findings, we speculate that the EMT-inducer ZEB1 may support tumor metastasis in B16F10 melanoma-bearing mouse model by promoting tumor cell mobility and dissemination, and that antitumor immune responses induced by tumor vaccine B16F10/GPI-IL-21 might result in tumor cell apoptosis and inhibit tumor growth but do not block the EMT of B16F10 cells or metastasis in mice. This evidence concerns the gene ZEB1 and melanoma.