According to multiplex PCR, all 135 screened samples were negative for the most frequent PGF of B-lineage ALL: TEL-AML1, E2A-PBX, MLL-AF4, and BCR-ABL (p190) and for the most frequent PGF of acute myeloid leukemia (AML): AML-ETO, PML-RARA, and CBFβ-MYH11. Here, AFF1 is linked to acute myeloid leukemia.