CFTR and cystic fibrosis: Subsequently the CFTR allelic variant 186-8T/C (c.54-8 T/C) in intron 1 of CFTR gene was identified by gene sequencing in an Italian CF Center where genetic analysis was performed by reverse dot blot (INNO-LIPA CFTR19, CFTR17 + TN Update, CFTR Italian Regional), sequencing and Multiplex ligation-dependent probe amplification (MLPA).