The immunohistochemistry analysis of tumor section by H&E, and by proliferation antigens against PCNA staining revealed that thiacremonone inhibited tumor growth, but assessments of pro-apoptotic proteins, Bax and cleaved caspase-3 by IHC revealed more frequently in thiacremonone-treated LLC bearing C57BL/6J mice and PRDX6 overexpressed mice (Fig. 6C and D). This evidence concerns the gene CASP3 and neoplasm.