Additional analysis of a larger set of individual samples in combination with more traditional immunoassays such as ELISA are required to further confirm whether high level of serum alpha-2-HS-glycoprotein precursor and β-tubulin increased odds ratios (ORs) of CRC in a nested case–control sample of CRC individuals such as those observed in this study. The gene discussed is AHSG; the disease is colorectal carcinoma.