Dynamic alterations in differential protein expression related to HCC invasion (e.g. ICAM-1, ANXA1, CK18, FTL, GSN, HSP90, PCNA, Vimentin, PHB2, Clusterin, S100A11, and Vitronectin) were verified by Western blot (Fig. 7A) and quantitative RT–PCR (Fig. 7B), respectively. The gene discussed is ANXA1; the disease is hepatocellular carcinoma.