In support, another report showed a key role of cytochrome c release from the mitochondria in the pathogenesis of ischemia/reperfusion injury by examining cytochrome c in the mitochondria and cytosol, suggesting that the myocardioprotective effects of isoflurane preconditioning were associated with inhibition of cytochrome c loss from mitochondria [26]. This evidence concerns the gene CYCS and ischemia.