INS and Insulin resistance: Serum insulin concentrations weredetermined by ELISA kit (Mercodia, Uppsala, Sweden).Hemoglobin A1C (HbA1C) levels were measuredby enzymatic and chromatographic methodsusing commercial kits (Bio-system S.A., Germany).Insulin resistance was determined by calculating thehomeostasis model assessment of insulin resistance(HOMA-IR) score using the following formula (8):