FOS and Anxiety: The ANCOVA for MT-Fos colocalization that includes Behavioral PC3 (Anxiety) as a covariate yields only a significant main effect of Sex [F(1, 32) = 4.13, p = 0.05], whereas the parallel analysis of MT-Fos double-labeled neuron numbers yields a significant Sex × Context interaction [F(1, 32) = 5.63, p < 0.02] that is virtually identical to the one shown in Figure 7, which includes Behavioral PC1 as the covariate.