We tested A549 human NSCLC cells which reportedly express Shh [10], [37]; to test if Shh produced by A549 cells was able to induce a Hh response in surrounding cells in a juxtacrine/paracrine fashion, A549 cells were co-cultured with C3H10T1/2 cells. The gene discussed is SHH; the disease is non-small cell lung carcinoma.