The C3H10T1/2 cell response by ALP production was reduced by 37±10% (p = 0.036) compared to treatment with a non-targeting pool of siRNAs (Figure 6C), showing that Hhat KD indeed leads to a reduction in juxtacrine/paracrine Shh signaling by A549 NSCLC cells. This evidence concerns the gene SHH and non-small cell lung carcinoma.