CD4+ and CD8+ T lymphocytes were detected by flow cytometry in the tumor tissues of the mice immunized with the plasmids containing the fusion antigen gene fragments (PSVK-shFcG-GM/B7.1 group and PSVK-shFcG group), but the corresponding cell populations were not detected in the other groups (both immunization strategies; Fig. 5a: prevention model; Fig. 5b: therapeutic model). Here, CD80 is linked to neoplasm.