This study has profiled the expression of the retinoic acid metabolising enzymes CYP26A1, CYP26B1, CYP26C1 and LRAT using a well characterised colorectal cancer tissue microarray with monoclonal antibodies to CYP26A1, CYP26B1, CYP26C1 and LRAT respectively, that have been developed and characterised for their use by immunohistochemistry on formalin fixed wax embedded tissue. This evidence concerns the gene CYP26B1 and colorectal cancer.