The characteristic subcellular distribution and staining intensity of these analytes in immunostained ccRCC tumors dictated how EC were assessed for their presence: Ki67 and p-STAT3 are nuclear, and their expression was assessed by quantifying staining within nuclear boundaries drawn during segmentation; p-ERK is found in the nucleus, cytoplasm or both and was assessed by quantifying staining within a perimeter of two pixels (0.5 micron in images taken at 400X magnification) beyond nuclear boundaries. This evidence concerns the gene MKI67 and nonpapillary renal cell carcinoma.