CSTB and Brain atrophy: In order to clarify the spatiotemporal progression of the brain pathology, this study aims to quantify the dynamics of brain atrophy and WM changes in Cstb-/- mice from 1 month of age up to the fully symptomatic age of 6 months using in vivo magnetic resonance imaging (MRI) for detecting volumetric changes and ex vivo diffusion tensor imaging (DTI) with tract-based spatial statistics (TBSS) analysis for detecting changes in WM integrity [14].