To model clinically relevant scenarios we designed treatment protocols in the ECM model whereby Plasmodium berghei ANKA (PbA)-infected mice were treated with a combination of artesunate (10 mg/kg), and a PPARγ agonist (rosiglitazone; 2.5 mg/kg) or saline as a control starting on day 3 post-infection, when parasitemia becomes detectable peripherally, or when mice displayed neurological symptoms and began to die from CM (between day 5 and 6 of infection) (see Figure S1 for treatment protocols). This evidence concerns the gene PPARG and infection.