Furthermore, as shown in Table 3, CCK8 analysis demonstrated that the incubation of leukemia cells with LY294002 or rapamycin strongly increased the sensitivity of leukemia cells to 100 nM or 1 μM of mitoxantrone (P<0.05), suggesting that chemosensitivity to mitoxantrone is tightly correlated with decreased p-PI3K and p-Akt expression in the resistant K562 cells. The gene discussed is AKT1; the disease is leukemia.