NANOG and cancer: Taken together, these results suggest that 1)the rNanogP8 proteins, like endogenous Nanog1 and rNanog1 in NTERA-2 cells, seem to be able to adopt different conformations that exhibit varying mobility on reducing SDS-PAGE; 2)somatic cancer cells may encode different ‘isoforms’ of NanogP8 due to sequence polymorphisms (such as those in HPCa6 and MCF7 cells); and3)NanogP8 proteins of different conformations or polymorphisms may be differentially recognized by various anti-Nanog Abs with the 42 kD still being the major band on WB.