First, at the mRNA level, rigorous studies employing differential RT-PCR combined with sequencing or differential sensitivity to the restriction enzyme AlwN1 [15], [17], [31], [32], [34], [46], have demonstrated that somatic cancer cells preferentially express the transcript of the NanogP8 gene (Fig. S1B; see below) rather than Nanog1. Indeed, we have observed that the nanog1 locus is silenced in some somatic cancer cells [15]. The gene discussed is NANOGP8; the disease is cancer.