We conclude, that no effective replication of SeV FmutYko took place in non-malignant cells, which was in clear contrast to the production of a high amount of viral progeny in hepatoma cells (mean peak titer for PLC/PRF/5, Hep3B and HuH7 hepatoma cells over the same collection period: 1.8×107 TCID50). This evidence concerns the gene HSPG2 and hepatocellular carcinoma.