We used translational blocking and splice blocking morpholinos for knocking down ttc26. Both morpholinos effectively suppressed TTC26 expression (Figure 2—figure supplement 1A ) and caused phenotypes that are typical of defective cilia in zebrafish (Malicki et al., 2011), including left–right asymmetry defects with abnormal heart looping (Figure 2A,B), as well as hydrocephalus, pronephric cysts, abnormal ear otolith formation, and curly body axis (Figure 2—figure supplement 1B,C). Here, IFT56 is linked to Hydrocephalus.