TGFBI and osteosarcoma: To test the role of βig-h3 in human osteosarcoma cells, small interfering RNAs against βig-h3 (βig-h3 siRNA) were transfected into the human osteosarcoma cell lines, Saos-2 cells and MG63 cells, for 48 hours to knockdown βig-h3 mRNA and protein expression.