We considered CD31, CD8 (cell surface epitopes) and FOXP3 (intracellular epitope) as immunovascular biomarkers of major interest in osteosarcoma, representing intratumoral vessels (CD31), tumor attacking T-cells (CD8) and immunosuppressive or anergic T-cells (FOXP3). This evidence concerns the gene FOXP3 and osteosarcoma.