Moreover, some studies suggest that the antitumor effect of CTLA-4 blockade might depend on the depletion of Treg [29], as demonstrated in a model of mouse melanoma, in which both the enhancement of T effector cell function and inhibition of Treg activity through the blockade of CTLA-4 led to a strong antitumor response [25]. The gene discussed is CTLA4; the disease is melanoma.