Furthermore, Diaz et al. have used coimmunoprecipitation and plasmin activity assays to demonstrate that ANXA2 specifically binds to t-PA on the extracellular membrane of pancreatic cancer cells (PANC-1 and SK-PC-1 cells), where it activates local plasmin production and tumor cell invasion [88]. This evidence concerns the gene ANXA2 and familial pancreatic carcinoma.