Animal models have suggested that the onset of arthritic disease is preceded by phenotypic changes in draining lymph nodes (LN).64, 65, 66 Flow cytometry data in LN biopsies from ACPA positive at risk individuals, early arthritis patients and healthy controls suggested increased T-cell activation in early arthritis but not in ACPA-positive individuals.67, 68 These data support the rational for further extensive molecular analysis of LN during different phases of (preclinical) arthritis. The gene discussed is PRTN3; the disease is Arthritis.