In the present study, we identified eEF2 as an antigen eliciting humoral immune responses in a group of patients with HNSCC or colorectal cancer by immunoblot analysis and showed that eEF2 was overexpressed in the majority of various types of cancers such as lung, esophageal, pancreatic, breast and prostate cancers, HNSCC, glioblastoma multiforme and NHL. The gene discussed is EEF2; the disease is colorectal cancer.