SIRT1 and obesity due to melanocortin 4 receptor deficiency: Because it may be thought to affect the CVD phenotype by associating body mass index and obesity, glucose tolerance and diabetes, blood pressure, cholesterol metabolism and coronary artery calcification, we performed our experiments on rs7895833 A>G in the promoter, rs7069102 C>G in intron and rs2273773 C>T in exon regions of SIRT1 gene.