We did not measure protein turnover (synthesis/degradation) or energy expenditure of mice in the present study but, if we accept that reduced mTORC1 pathway activation reflects reduced protein turnover, then we might speculate that a concomitant reduction in basal energy expenditure could be a contributing factor in the development of insulin resistance in MS-Slc7a5-KO mice on a high-protein diet. This evidence concerns the gene SLC7A5 and Insulin resistance.