The 5X Familial Alzheimer’s Disease (5XFAD) mouse line co-overexpresses APP with three FAD mutations (K670N/M671L, I716V, and V717I) and PS1 with two FAD mutations (M146L and L286V) under the control of the neuron-specific thy1 promoter [20]. Here, PSEN1 is linked to early-onset autosomal dominant Alzheimer disease.