In summary, these results indicate that concomitant PD-1 blockade and OX40 triggering creates higher ratios of effector T cells to immunosuppressive cells in peritoneal cavity of treated mice, which represents the shift of the normally suppressive tumor milieu to a more stimulatory state which is more permissive for immune mediated tumor destruction. This evidence concerns the gene TNFRSF4 and neoplasm.