As ID8 cells express the mesothelin, a well-characterized tumor antigen [38], we harvested splenocytes from treated mice, and the same number of splenocytes was cultured in the presence of 10 μg/mL of H-2Db-restricted mesothelin-specific peptide (amino acid 406–414) or control HPV-E7 peptide (amino acids 49–57) for 3 days and analyzed IFN-γ production in culture supernatants by ELISA. Here, MSLN is linked to neoplasm.