To determine the molecular basis of how TSPY1 enhanced invasive abilities of HCC cells, we next examined two invasion-related genes CXC chemokine receptor 4 (CXCR4) and hypoxia inducible factor-1 (HIF-1) that are known to play major role in tumor metastasis. The gene discussed is CXCR4; the disease is hepatocellular carcinoma.