Using scanning electron microscopy, we evaluated the morphology (Figure 1A, B, and C) of MDA-MB-231 human breast tumor cells before (MDA-231WT) and after (MDA-231MYOF-KD) stable lentivirus-mediated transduction of shRNAs targeting MYOF as well as in a control line expressing a non-human, non-targeting shRNA (MDA-231LTVC). Here, MYOF is linked to breast neoplasm.