We further validated the effect on tumor invasion in vivo: tumors formed by miR-100-overexpressing HMLE-Erbb2 cells were well demarcated and did not show overt invasion to their surrounding tissues (Figure 5F); in contrast, tumors formed by either the control HMLE-Erbb2 cells (mock) or HMLE-Erbb2 cells with simultaneous expression of miR-100 and HOXA1 were invasive and infiltrated muscular, adipose and stromal tissues (Figure 5F). Here, HOXA1 is linked to neoplasm.