IFN-γ production and proliferation of memory cells are two major readouts that have been used to demonstrate cross-reactivity [34], [59], [60], [66], and LCMV memory cells did not produce IFN-γ in response to restimulation with leishmanial antigen and did not proliferate in response to infection with L. major (Figure 2F and S3). This evidence concerns the gene IFNG and infection.