To determine if IFNβ completely abrogated MCMV IE gene expression, LSECs were infected with MCMVr in 96-well plates, scanned for reporter gene expression, and wells were classified as positive when even a single EYFP-fluorescent cell could be observed within a week of infection (representative result of an experiment in 12 wells per condition is shown in Figure 3B). Here, IFNB1 is linked to infection.