In order to investigate the effect of NF-κB/IκB on phenotypic transdifferentiation of Fb towards MF and the inbibitional effect of p65 antisense oligonucleotide on NF-κB in that transdifferentiation in pathological process of IPF, this experiment was performed. Here, NFKB1 is linked to idiopathic pulmonary fibrosis.