AKT1 and neoplasm: Densitometry of the IHC images showed a significant reduction in the expression of pAkt S473 in treated animals as compared to control animals (p < 0.02) as shown in Figure 4B. The loss of phosphorylation of Akt was further confirmed by western blot analysis of MK-2206-treated tumor tissue lysates showing a reduction in pAkt at both S473 and T308 sites, in comparison to the control xenograft tumors (Figure 4C).